원문정보
초록
영어
Hot water extracts from 16 domestic edible mushrooms including Pleurotus ferulae (Lanzi) X.L. Mao, Lentinula edodes (Berk.) Pegler, and Hypsizygus marmoreus (Peck) H.E. Bigelow, which are commercially available, were used for determining the cosmetic potential of these mushrooms. In this study, we carried out in vitro functional experiments to determine the inhibitory effects of these extracts on L-DOPA oxidation of tyrosinase and melanin synthesis. Based on the results of these experiments, H. marmoreus (Peck) H.E. Bigelow No. 10 and No. 15 were selected for further analysis. We analyzed the melanin synthesis inhibitory activity, TRP1 and MITF expression via real-time PCR, and Fontana Masson staining in artificial skin Neoderm®-ME. Taken together, we observed that the hot water extract from H. marmoreus (Peck) H.E. Bigelow (No. 15) had better whitening effect than the extracts of other mushrooms. Thus, it can be a potential source of skin-whitening agent.
목차
서론
재료 및 방법
버섯 열수 추출물 제조
세포주 배양
Cell viability 측정
L-DOPA oxidation inhibitory activity
Melanin synthesis inhibitory activity
In vitro test on Artificial skin
Melanin synthesis inhibitory activity
Real-time PCR
Paraffin block 제작
Fontana-Masson staining
Statistical analysis
결과 및 고찰
Cell viability
L-DOPA oxidation inhibitory activity
Melanin synthesis inhibitory activity
In vitro test on Artificial skin
적요
참고문헌