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Purpose：The aim of this study was to investigate the effects of stepper exercise using lower extremity patterns of PNF(Proprioceptive neuromuscular facilitation) on ankle muscle strength and balance ability of females in their twenties. Method：Twenty subjects participated in this experiment were carried out the exercise program dose of 5 days per week for 2 weeks. They were divided into two groups; using twist stepper group(lower extremity patterns of PNF group, n=10) and using straight stepper group(n=10). The effects of twist stepper and straight stepper were evaluated by measurements of normal standing(NSEO, NSEC), and dynamic type 1 and dynamic type 2, respectively. For each case, the experimental data were obtained about static balance in 3 items : mean X speed, mean Y speed and velocity moment, that of dynamic balance in 4 items : mean X speed, mean Y speed, time, distance. Results：The results of this study were as follows; 1) In straight stepper group, the statistically significants were significants were shown on right/left ankle muscle strength in case of dorsi-flexion. 2) In twist stepper group, the statistically significants were significants were shown on right/left ankle muscle strength in case of dorsi-flexion. 3) In straight stepper group, the statistically significants were significants were shown on right ankle muscle strength in case of plantar-flexion. 4) In twist stepper group, the statistically significants were significants were shown on right/left ankle muscle strength in case of plantar-flexion. Conclusion：The above results revealed that twist stepper exercise using lower extremity patterns of PNF was more effective than straight stepper exercise for improving the ankle muscle strength.