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PURPOSE : The purpose of this study was to compare effects of isometric hip adduction using tools on muscle activity rates of VMO and VL while performing squat. METHOD : Seventeen healthy male subjects, between twenty and fourty years old, performed traditional squat exercise and squat with isometric hip adduction using ball, Theraband, Pilates circle on stable and unstable base of support(BOS) for 3 sets of 5 seconds. They had a rest for 30 seconds between each set and rest for 30 minuts between each tool. The muscle activities of VMO and VL while performing the exercise were measured by electromyography(EMG) and muscle activity rates of VMO and VL was calculated. The obtained data were analyzed using SPSS 23.0 version. RESULT : Squat with isometric hip adduction using ball produced significantly high muscle activity rate of VMO(p<0.001). Muscle activity rate of VMO during performing squat with isometric hip adduction using tools and traditional squat was significantly greater on stable BOS than on unstable BOS. CONCLUSION : The exercise on unstable surface is more effective than on unstable surface. The ball squat exercise is the most effective than another 3 exercise group(TS, TS, PS). The best method for the selective strengthening of VMO muscle is ball squat exercise on unstable surface.