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논문검색

한우 GPD1 유전자의 SNP와 육량 및 육질형질과의 연관성 분석

원문정보

Association Between the Single Nucleotide Polymorphism of Glycerol-3-phosphate Dehydrogenase 1 Gene and Meat Yield and Quality Traits in Hanwoo (Korean Cattle)

김준성, 방주희, 신성철, 정의룡

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초록

영어

This study aimed to indentify single nucleotide polymorphisms (SNPs) in exon region of the glycerol-3-phosphate dehydrogenase 1 (GPD1) gene and to evaluate their associations with meat yield and quality traits in Hanwoo (Korean cattle). Two SNP markers, g.2766C>T and g.5105A>G were identified in the exons 5 and 8, respectively. Genotyping of the two SNPs was carried out using PCR-RFLP analysis in 309 Hanwoo steers to evaluate their associations with meat yield and quality traits. As a result, g.2766C>T in exon 5 was significantly associated with carcass weight (CW) and marbling score (MS). Animals with the CC genotype of g.2766C>T had heavier CW than those with the CA or AA genotype. Animals with the CC genotype of g.2766C>T also had higher MS than those with the CA or AA genotype. Additive effect was also observed with CW and MS traits. We constructed haplotypes by linkage disequilibrium analysis and analyzed association between haplotypes and meat yield and quality traits. Haplotype of GPD1 gene was associated with CW. As a result, animals with CA haplotype had heavier CW than TG haplotype. These findings suggest that the SNPs of bovine GPD1 gene may be a useful molecular marker for selection of meat yield and quality traits in Hanwoo

목차

ABSTRACT
 Ⅰ. 서론
 Ⅱ. 재료 및 방법
  1. 공시재료 및 genomic DNA 분리
  2. 한우 GPD1 유전자의 SNP 탐색 및 검출
  3. 한우 GPD1 유전자의 SNP genotyping
  4. 한우 GPD1 유전자의 SNP와 육량 및 육질형질과의 연관성 분석
  5. 한우 GPD1 유전자의 haplotype과 육량 및 육질형질과의 연관성 분석
 Ⅲ. 결과 및 고찰
  1. 한우 GPD1 유전자의 SNP 검출
  2. 한우 GPD1 유전자의 SNP genotyping
  3. 한우 GPD1 유전자의 SNP marker와 한우 육량 및 육질과의 연관성 분석
  4. 한우 GPD1 유전자의 haplotype과 육량 및 육질형질과의 연관성 분석
 Ⅳ. 요약
 사사
 Ⅴ. REFERENCES

저자정보

  • 김준성 Jun Sung Kim. 상지대학교 동물생명자원학부
  • 방주희 Ju Hee Bang. 서울대학교 의과대학 암연구소
  • 신성철 Sung Chul Shin. 상지대학교 동물생명자원학부
  • 정의룡 Eui Ryong Chung. 상지대학교 동물생명자원학부

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자료제공 : 네이버학술정보

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