earticle

영어

Purpose: This present study was performed to investigate anti-oxidant and anti-bacterial activities of Arecae pericarpium MeOH extracts and its solvent fractions. Methods: Arecae pericarpium was extracted by 80% MeOH and fractionated by n-hexane, ethyl acetate (EtOAc), n-butanol, and H2O. Free radical scavenging activities on 80% MeOH Arecae pericarpium extracts and its fractions were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Anti-oxidant effects were confirmed by catalase (CAT) and superoxide dismutase (SOD) activities. Anti- bacterial activity was evaluated by the paper disk diffusion method. Results: Extraction yields of 80% MeOH Arecae pericarpium extracts and its fractions such as n-hexane, EtOAc, n-butanol, and H2O, were 19.04, 14.33, 12.07, 22.53, and 50.97%, respectively. The 80% MeOH extracts got the highest value (16.6 mg/g) in total polyphenol contents. In the case of DPPH free radical scavenging activity, the n-hexane fraction was 102.9 mg/mL. It was similar to the value of positive control, vitamin C and butylated hydroxytoluene (BHT). The highest values of SOD and CAT activities were confirmed at the EtOAc fraction (38.73 U mg protein-1) and the n-butanol fraction (2.75 U mg protein-1 min-1), respectively. In the results of anti-bacterial activity, 80% MeOH Arecae pericarpium extracts had anti-bacterial effects on all eight microorganisms. The n-hexane fraction had the anti-bacterial effects on only Listeria monocytogenes. There are no anti-bacterial effects on the other fractions such as EtOAc, n-butanol, and H2O. Conclusion: It is considered that these results imply the 80% MeOH Arecae pericarpium extracts can be used as not only natural antioxidants but also natural antibacterials.

한국어

목적: 본 연구는 대복피 메탄올(methanol, MeOH) 추출물 및 용매 분획물의 항산화 및 항균활성을 확인하고자 수행되었다. 방법: 대복피를 80% MeOH로 추출하고, n-hexane, ethyl acetate (EtOAc), n-butanol, H2O로 분획하였다. 추출물과 분획물의 추출 수율, 총 폴리페놀 함량을 측정하였다. 대복피의 80% MeOH 추출물과 용매 분획물의 free radical 소거능은 1,1-diphenyl- 2-picrylhydrazyl (DPPH) 방법에 의해 평가하였다. 항산화 활성은 superoxide dismutase (SOD) 및 catalase (CAT) 활성으로 평 가하였다. 항균활성은 디스크 확산법에 의해 평가하였다. 결과: 대복피 80% MeOH 추출물 및 n-hexane, EtOAc, n-butanol, H2O 용매 분획물의 추출 수율의 결과, 각각 19.04, 14.33, 12.07, 22.53, 50.97%로 측정되었다. 총 폴리페놀 함량은 80% MeOH 추출물에서 16.6 mg/g으로 가장 높게 측정되었다. DPPH free radical 소거능은 n-hexane 분획물에서 102.9 mg/mL로 양성대조 군인 vitamin C 및 butylated hydroxytoluene (BHT)와 비슷한 우수한 항산화 활성을 나타내었다. SOD 활성은 EtOAc 분획물에서 가장 높은 값(38.73 U mg protein-1)을 나타내었다. CAT 활성은 n-butanol 분획물에서 가장 높은 값(2.75 U mg protein-1 min-1) 을 나타내었다. 항균활성의 결과에서, 대복피 80% MeOH 추출물은 8종의 균주에 대해 항균활성을 나타냈다.- nHexane 분획물은 오직 Listeria monocytogenes에 대해서만 항균활성을 나타냈다. EtOAc, n-butanol, H2O 분획물에서는 8종 균주에 대하여 항균 활성을 나타내지 않았다. 결론: 이상의 결과에서 대복피 80% MeOH 추출물은 천연 항산화 및 항균 소재로서의 활용이 가능할 것으로 기대된다.

중국어

目的: 探索槟榔陈皮甲醇提取物和不同溶剂萃取物的抗氧化及抗菌活性研究。方法: 槟榔陈皮用 80% MeOH提取，并分 别用不同溶剂萃取相，即，n-hexane、ethyl acetate (EtOAc)、n-butanol、H2O依次萃取。测定 甲醇提取率和不同溶 剂萃取率，并测定总多酚含量。槟榔陈皮甲醇提取物和溶剂萃取物的自由基清除能力用1,1- diphenyl-2-picrylhydrazyl (DPPH)方法来评价。抗氧化活性用superoxide dismutase (SOD)和catalase (CAT)来评价。抗菌 活性用纸片扩散法 (paper disk diffusion method)来评价。结果: 80% MeOH提取率为19.04%，不同溶剂萃取率，即 ，n-hexane、EtOAc、 n-butanol、H2O溶剂的萃取率分别为14.33，12.07，22.53，50.97%。总多酚含量在80% MeOH提 取物中，以16.6 mg/ g显示最高值。DPPH 自由基消除能力在 n-hexane溶剂萃取物中，以102.9 mg/mL与良性对照群 vitamin C和butylated hydroxytoluene (BHT) 有相似的抗氧化活性。SOD活性在EtOAc溶剂萃取物中，以38.73 U mg protein-1显示最高值。CAT 活性在 n-butanol溶剂萃取物中以2.75 U mg protein-1 min-1 显示最高值。在抗菌活性评价结果中 ，80%甲醇提取物对8种 微生物具有抗菌活性，但是n-hexane萃取物只有对Listeria monocytogenes 菌具有抗菌活性，其 他EtOAc、n-butanol、 H2O溶剂萃取物对8种微生物都不具有抗菌活性。结论: 从以上的结果中得出，80%甲醇提取物可以 充分利用于天然抗氧 化及抗菌活性原料。