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The purposes of this study are to investigate university students’ emotional responses and the characteristics regarding lecture voice preference. 45 years old female professor’s lecture voice samples were collected with laryngitis and normal condition. 66 female university students who majored in speech language pathology and early childhood education listened the lecture voice samples, and then filled the Adjective Checklist and responded questionnaire items. As a result, the university female students had negative emotions to hoarse lecture voice sample and positive emotions to normal lecture voice sample. And they showed significant higher scores related to unfavorable lecture voice characters after listening to a hoarse lecture voice sample. These results suggested that the importance of professor’s voice quality and necessity of controlling voice problems.