원문정보
초록
영어
Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is one of the important diseases in many countries. In the previous study, four rhizobacterial strains were selected which were shown disease suppression after its pre-treatment on the citrus leaves following inoculation with Xcc. In order to illustrate the mechanism mediated by these selected rhizobacterial strains, the citrus leaves pre-treated with the selected strains was investigated after inoculation with Xcc. The number of Xcc from the rhizobacterial strains pre-treated leaves was effectively reduced compared to that of untreated control one, indicating the direct suppression by the selected rhizobacterial strains on the citrus leaf surface. From the scanning electron microscopical images, it revealed that the number of total bacterial cells on the leaves pre-treated with the rhizobacterial strains was reduced compared to those on the untreated leaves. Based on these results, it was suggested that citrus canker could be suppressed by the rhizobacteria which might cause direct anti-bacterial activity on the surface of citrus leaves.
목차
Introduction
Materials and Methods
Plants
Inoculums and rhizobacterial strains
Pre-treatments with rhizobacteria orStreptomycin®
Inoculation with Xcc on the leaves of branches
Determining the density of Xcc on thecitrus leaves pre-treated withrhizobacterial strains or Streptomycin®
Identification of Xcc or rhizobacteiral strains
Scanning electron microscopicobservation of bacteria on the citrus leaves
Statistical analyses
Results
Suppression of disease severity by the pre-treatment with rhizobacterial strains
Reduction of number of Xcc on the leaves pre-treated with rhizobacterial strains
Identification of Xcc or rhizobacteria on the semi-selective medium
Scanning electron microscopy on the citrus leaves pre-treated the rhizobacteria
Discussion
Acknowledgement
Referances