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Purpose: The purpose of this cross-sectional study was to describe smoking characteristics, and then to examine relationships between self-efficacy for smoking cessation and preparation of smoking cessation in service and sales woman employee with smoking in department stores. Methods: This study was a secondary data analysis carried by the smoking cessation support center. One hundred six employees smoking women participated from two department stores. Collected data were analyzed using SPSS 20.0 program. Descriptive statistics, t-test, one-way ANOVA along with Scheffé test, pearson correlation coefficients were used for statistics. Results: The study results have shown that the mean score of self-efficacy for smoking cessation 5.1±2.62, preparation smoking cessation 4.0±2.89. There was positive relation between self-efficacy for smoking cessation and preparation smoking cessation. Conclusion: Considering the findings from this study, there is a need to develop self-efficacy for smoking cessation program for effective smoking cessation in service and sales woman smokers employee.