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Objectives: The purpose of this study was to examine the relationships of the drinking and smoking behaviors of health science majors to their knowledge of and attitude toward drinking and smoking in an effort to determine the influential factors for their drinking and smoking. Methods: The subjects in this study were 380 selected health science majors in two four-year universities and a three-year college that were respectively located in Seoul and Chungcheong Province. A survey was conducted with structured questionnaires from September 1 to 15, 2015. As for statistical data analysis, a statistical package SPSS 18.0 was utilized. Results: There were significant differences between the male and female students in smoking(p=0.000), and no gender differences were found in drinking(p=0.754). The male students who both drank and smoked outnumbered the female students who did, and the female students who only drank outnumbered the male students who did. And the differences were statistically significant(p=0.000). There were statistically significant differences according to smoking and drinking in relationship with professors(p=0.020), peer relationship(p=0.035), drinking attitude(p=0.033, smoking knowledge(p=0.008) and smoking attitude (p=0.006). The factors that affected drinking were religion and the drinking of fathers, and the factors that exerted an influence on smoking were gender, academic year, form of residence, religion and the smoking of family. Conclusions: A lot of temperance and antismoking education programs should be provided for college students in their early school years in the form of peer teaching and in association with their major classes. Besides, they should be encouraged to join clubs related to temperance and antismoking, and professors should try to facilitate their relationship building.