원문정보
SNPs in Promoter Region of Hanwoo ANK1 Gene with Their Effects on Economic Traits in Hanwoo
초록
영어
The ANK1 (Ankyrin-1) gene, located on the bovine chromosome 27, encodes a structural protein which forms an important component of the cytoskeleton. Ankyrin belongs to a protein family that links membrane proteins to the underlying spectrin-actin cytoskeleton. Many studies on gene expression regulation have revealed that RNA polymerase binds to the ANK1 gene promoter region. The purpose of the present study was to investigate the relationship between the SNP of the ANK1 promoter region and economic traits in Hanwoo cattle. A total of seven SNPs (C-944T, C-733T, C-687G, A-672G, C- 307T, A-104G, C-24T), found in 119 animals, were correlated to economic traits. One of these SNPs, A-104G, was reported for the first time in the present study. Three newly discovered haplotypes were not associated with economic traits. Significant (p<0.05) relationships were found between C-944T and carcass weight, backfat thickeness, loin muscle area and between C-733T, A-672G and intramuscular fat. These results suggest that the SNPs of ANK1 gene may be useful molecular markers for selection of meat yield and quality traits in Hanwoo
목차
Ⅰ. 서론
Ⅱ. 재료 및 방법
1. 공시재료
2. Genomic DNA 추출 및 quality control
3. Polymerase chain reaction
4. SNP 탐색 및 유전자형 결정
5. 통계분석
Ⅲ. 결과 및 고찰
1. ANK1 promoter 지역 내 SNP 탐색
2. ANK1 promoter 유전자형 분석
3. ANK1 promoter Haplotype 분석
4. ANK1 promoter SNP와 경제형질 연관성 분석
Ⅳ. 요약
Ⅴ. REFERENCES