원문정보
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초록
영어
The peptide sequences, GHK(Gly-His-Lys) and KTTKS(Lys-Thr-Thr-Lys-Ser), using a collagen stimulator recently were manipulated at N-terminal as a multifunctional peptide derivative with PEG (polyethyleneglycol) linker connected to gallic acid which presents anti-inflammatory activity. The multifunctional peptide derivatives were obtained in a normal peptide preparation method through SPPS(solid phase peptide synthesis) using Fmoc chemistry and a carboxyl group insertion reaction of PEG-3,4,5-triacetoxy benzoate by using potassium tert-butoxide and ethyl bromoacetate, which was separated by Sephadex DEAE. It gave a good compromise to a cosmetic application for cell cytotoxicity, anti-wrinkle, and anti-inflammation.
목차
Abstract
1. Introduction
2. Experiments
2.1. Reagents and Instrument
2.2.1. Synthesis of Protected Peptide Segment(GHK, 1a)
2.2.2. Synthesis of Protected Peptide Segment(KTTKS, 1b)
2.3. Synthesis of 3,4,5-triacetoxy Benzoic Acid
2.4. Synthesis and Purification of Partially Oxidizedα-Hydroxy-ω-carboxyl Polyethylene Glycol (HO-PEG-COOH)
2.5. Synthesis of PEGylated 3,4,5-Triacetoxy-benzoic Acid (2)
2.6.1. Synthesis of PEGylated GHK Derivative(3a)
2.6.2. Synthesis of PEGylated KTTKS Derivative(3b)
2.7. Preparation of Nano-structure
2.8. Measurement of Biological Activity
3. Results and Discussion
References
1. Introduction
2. Experiments
2.1. Reagents and Instrument
2.2.1. Synthesis of Protected Peptide Segment(GHK, 1a)
2.2.2. Synthesis of Protected Peptide Segment(KTTKS, 1b)
2.3. Synthesis of 3,4,5-triacetoxy Benzoic Acid
2.4. Synthesis and Purification of Partially Oxidizedα-Hydroxy-ω-carboxyl Polyethylene Glycol (HO-PEG-COOH)
2.5. Synthesis of PEGylated 3,4,5-Triacetoxy-benzoic Acid (2)
2.6.1. Synthesis of PEGylated GHK Derivative(3a)
2.6.2. Synthesis of PEGylated KTTKS Derivative(3b)
2.7. Preparation of Nano-structure
2.8. Measurement of Biological Activity
3. Results and Discussion
References
저자정보
참고문헌
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