원문정보
초록
영어
This study was designed to investigate the collagen metabolism and tyrosinase activity of Myrrh Extracts (ME). The currently known effects of ME are to move blood, calm pain, solve edema, and promote tissue regeneration. The effect of ME on type I procollagen production and collagenase (matrix metalloproteinase-1, henceforth referred as MMP-1) activity in human normal fibroblasts Hs68 after ultraviolet B (UVB, 312 nm) irradiation was measured using the ELISA method. The tyrosinase activity after treatment with ME was measured as well. There was no cytotoxicity at all tested concentrations. ME significantly inhibited the increased collagenase activity after UVB damage, whereas it did not recover the reduced type I procollagen production in UVB damaged Hs68 cells. It did not reduce the L-DOPA oxidation. However, it significantly reduced the tyrosinase activity. In conclusion, ME showed the anti-wrinkle effects via the collagenase inhibitory mechanism and whitening effects via the tyrosinase inhibitory mechanism. Although further research is needed to validate its efficacy, these results suggest that ME may have potential as an anti-aging ingredient in cosmetic herb markets.
목차
1. Introduction
2. Methods
2.1. Sample Preparation
2.2. Reagents
2.3. Cell culture
2.4. UVB Irradiation
2.5. Cell Viability
2.6. Assays of Collagen Type I Synthesis and Collagenase Inhibition
2.7. Tyrosinase Inhibition Assay
2.8. Inhibition of L-DOPA Oxidation
2.9. Statistical Analysis
3. Results
3.1. Cytotoxicity on Hs68 Human Fibroblasts
3.2. Assay of Collagen Type I Synthesis
3.3. Assay of Collagenase Activity
3.4. Inhibitory Effects on Tyrosinase Activity
3.5. Inhibitory Effects on L-DOPA Oxidation
4. Discussion
5. Conclusions
Acknowledgments
References