원문정보
초록
영어
This study was conducted to screen and determine the antibacterial effects of soil bacterial isolates from three sampling sites of Sinapsapan, Jordan, Guimaras against Staphylococcus aureus and Escherichia coli. Test bacteria were isolated and characterized based on colonial morphology and cell characteristics. Soil bacteria isolates were screened for their antibacterial properties against S. aureus and E. coli using agar disc diffusion method. Each treatment was done in three replicates and trials. There were six bacterial isolates in each sampling site obtained and exhibited antibacterial properties on S. aureus and E. coli after 72 hours of incubation. For S. aureus, I4 (M=35.33 mm, active) from high sampling site; I2 (M=29.44 mm, partially active) from intermediate sampling site, and I1 (M = 38.00 mm, active) from low sampling site while for E. coli, I4 (M= 34.22, active) from high sampling site, I6 (32.78 mm, active) from intermediate sampling site, and I5 (M=34.78, active) from low sampling site had the highest zones of inhibition.
목차
1. Introduction
1.1 Background of the Study
1.2. Objectives of the Study
2. Methodology
2.1 Sampling Site
2.2 Soil Sample Collection
2.3 Isolation and Culture Condition
2.4 Test Organisms
2.5 Broth Culture of Test Isolates and Test Pathogens
2.6 Preparation of Antibiotic Solution
2.7 In vitro Screening of Soil Bacterial Isolates for Anti-bacterial Activity
2.8 Gram Staining of Bacterial Isolates with Antibacterial Properties
2.9 Data Collection Procedure and Analyses
3. Results
3.1 Bacterial Isolates in the Three Sampling Sites of Sinapsapan, Jordan, Guimaras
3.2. Characteristics of Bacterial Isolates in the Different Sampling Sites in Barangay Sinapsapan, Jordan, Guimaras
3.3 Zone of Inhibition of Bacterial Isolates in the Three Sampling Sites against Staphylococcus aureus BIOTECH 1582
3.4. Zone of Inhibition of Bacterial Isolates in the Three Sampling Sites AgainstEscherichia coli BIOTECH 1634.
3.5. Inferential Data Analysis
4. Discussion
5. Conclusions and Recommendations
Acknowledgement
References