원문정보
초록
영어
Cerebral ischemia is caused by reduced cerebral blood flow due to a transient or permanent cerebral artery occlusion. Ischemic injury in the brain leads to neuronal cell death, and eventually causes neurological impairments. Cordyceps, the name given to the fungi on insects, has abundant useful natural products with various biological activities. Cordyceps is known to have nephroprotective, hepatoprotective, an-ti-inflammatory, antioxidative, and antiapoptotic effects. We investigat-ed the effects of Cordyceps on short-term memory, neuronal apoptosis, and cell proliferation in the hippocampal dentate gyrus following tran-sient global ischemia in gerbils. For this study, a step-down avoidance test, terminal deoxynucleotidyl transferase-mediated dUTP nick end label-ing assay, immunohistochemistry for caspase-3 and 5-bromo-2’-de-oxyu-ridine, and western blot for Bax, Bcl-2, brain-derived neurotrophic fac-tor (BDNF), and tyrosin kinase B were performed. In the present study, Cordyceps alleviated cerebral ischemia-induced short-term memory impairment. Cordyceps showed therapeutic effects through inhibiting cerebral ischemia- induced apoptosis in the hippocampus. Cordyceps suppressed cerebral ischemia-induced cell proliferation in the hippo-campal dentate gyrus due to the reduced apoptotic neuronal cell death. Cordyceps treatment also enhanced BDNF and TrkB expressions in the hippocampus of ischemic gerbils. It can be suggested that Cordyceps overcomes cerebral ischemia-induced neuronal apoptosis, thus facili-tates recovery following cerebral ischemia injury.
목차
INTRODUCTION
MATERIALS AND METHODS
Preparation of aqueous extract of Cordyceps
Animals and treatments
Induction of transient global ischemia
Step-down avoidance test
Tissue preparation
TUNEL staining
Immunohistochemistry for BrdU
Western blotting
Data analysis
RESULTS
Effect of Cordyceps on short-term memory in the step-down avoidance test
Effect of Cordyceps on TUNEL-positive cells in the hippocampal CA1 region
Effect of Cordyceps on caspase-3 expression in the hippocampal CA1 region
Effect of Cordyceps on cell proliferation in the hippocampal dentate gyrus
Effects of Cordyceps on Bax and Bcl-2 expressions in the hippocampus
Effects of Cordyceps on BDNF and TrkB expressions in the hippocampus
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES