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The aim of this paper is to develop an efficient and a powerful Matlab based graphical user interface to address the problem of image segmentation. We propose two approaches for segmenting images: An automatic marker controlled watershed segmentation for segmenting an entire image or a scene and a semiautomatic graph cut based segmentation using fixation points. Automatic Watershed segmentation with a Sobel edge detector is used to detect the gradient of an input image resulting in an image less sensitive to noise. To deal with the usual problem of over segmentation using watershed, marker controlled watershed transformation is applied further for segmenting an image. Fixation based graph cut segmentation allows the user to analyze the input image displayed on the screen and specify some hard constraints indicating the object of interest or target object by using the mouse interaction. Experiments are done on the publically available dataset and the results of the supervised evaluation methods are observed to be satisfactory and are demonstrated along with the manually segmented reference image or a ground truth image obtained from segmentation evaluation database