earticle

영어

The fluorescence polarization immunoassay (FPIA) as a preliminary test sometimes gives false positive results due to structural similarity between amphetamine-type stimulants (ATS). Thus, a rapid and easy HS (headspace)-SPME (solid-phase microextraction)-GC (gas chromatography)-MS (mass spectrometry) screening method was developed and validated for simultaneous detection of 8 ATS in human urine. These drugs include methamphetamine, amphetamine, MDMA (3,4-methylenedioxymethamphetamine) and MDA (3,4-methylenedioxyamphetamine) which are popular abused drugs and their metabolites in Korea. Phentermine, ephedrine, pseudoephedrine and norephedrine/norpseudoephedrine also show cross- reactivity by FPIA. The conditions of SPME extraction time, temperature and deposition time were optimized to yield the highest peak area. To prevent carryover, the fiber was baked out pre and post injection into GC/MS. The data was collected by extracted ion chromatogram for each drug from total ion chromatograms by full scan mode and calculated by peak area ratios. Amphetamine, methamphetamine, MDA, MDMA and phentermine showed good resolution with retention times, whereas ephedrine, pseudoephedrine and norephedrine/norpesudo-ephedrine were not detected up to 1 μg/mL. The calibration curve showed acceptable linearity for each drug with R2> 0.99. The results of the intra-and inter-day precision and accuracy were satisfactory: <15 % for precision and within ±10 % for accuracy at three different concentrations (0.167, 0.333, 1.00 μg/mL). Thus, HS-SPME-GC-MS has the advantages of an easy sample preparation with acceptable accuracy and precision for the simultaneous quantification. It will be a useful method for a simple and rapid screening analysis of urines for ATS abuse.