earticle

영어

In order to develop methods to measure dioxin-like compounds in human serum. In addition to measurement of dioxin-like compounds, we established the method for sampling and storage. The volume of serum for analysis dioxin-like compounds was about 30ml(70ml of whole blood). The serum were stored in refrigerator until analysis. Serum(20-30g) was treated for 4h with 2N KOH/EtOH solution. The solution was extracted with n-hexane and the hexane phase was treated with sulfuric acid. The hexane phase was concentrated and applied to columns packed with multi layer silica gel, AgNO3, alumina and active carbom column. The 6 kinds of purification Method were applied to determine Dioxin-like compounds. The recovery of the dioxin were high in method 1(treated with sulfuric acid and multi-layer silica gel column, alumina column, active carbon column). And method 4(only multi-layer silica gel column) can remove the matrix of blood serum as effectively as a multi-layer silica gel column.