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Purpose: This study was conducted to identify the influencing factors of job stress, self-efficacy, and organizational culture to presenteeism in clinical nurses. Methods: A descriptive correlation research design was used for the this study. The subjects were 245 nurses working at the hospital in S, A and C City, from August 1st to October 30th 2014. Data were analyzed using t-test, ANOVA, Scheffé’s test, stepwise multiple regression by SPSS 21.0 program. Results: Job impairment indicated a significant positive relationship to job stress (p=.009), health problem(p<.001), and indicated significant negative relationship to self-efficacy (p<.001), innovative-oriented (p<.001), affiliative-oriented (p=.002), task-oriented (p=.026) in organizational culture. Significant factors of influencing to presenteeism were self-efficacy (p<.001, β=-.363), culture of task-oriented (p<.001, β=-.248) in organizational culture, and health problem (p=.002, β=.187). Conclusion: Self-efficacy was defined as a variable to influence on presenteeism, and developing of strategies and program to strengthen self-efficacy and task-oriented of organizational culture in nursing is helpful to reduce the presenteeism of clinical nurses.