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Purpose: This study aimed to examine the activity of the shoulder flexor and extensor when hold-relax and contraction-relax techniques were applied with shoulder joint flexion. Methods: The subjects of this study were 15 healthy women. With the shoulder joint flexion at 0° and 90°, hold-relax and contraction-relax techniques were applied for the same submaximal resistance to measure the activities of the deltoid muscle anterior fiber, deltoid muscle posterior fiber, pectoralis major fiber, and latissimus dorsi muscle with surface electromyography. An independent t-test was conducted in order to compare activities of each muscle according to the two techniques. Results: When the hold-relax and contraction-relax techniques were applied with the shoulder joint flexion at 0°, the activities of the shoulder flexor and extensor were not significantly different, but the activity of the flexor was higher when the contraction-relax technique was applied than when the hold-relax technique was applied. When the hold- relax and contraction-relax techniques were applied with the shoulder joint flexed at 90°, the activities of the shoulder flexor and extensor were not significantly different, but the activity of the extensor was relatively higher than when the flexor was at 0° Conclusion: When the hold-relax and contraction-relax techniques were applied with the shoulder joint flexion at 0°, the activities of the shoulder flexor and extensor were not significantly different, but the activity of the flexor was higher when the contraction-relax technique was applied than when the hold-relax technique was applied. When the hold- relax and contraction-relax techniques were applied with the shoulder joint flexed at 90°, the activities of the shoulder flexor and extensor were not significantly different, but the activity of the extensor was relatively higher than when the flexor was at 0°.