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Background: The purpose of this study was to investigate the effects of Mulligan’s straight leg raise with traction technique and hold-relax technique on flexibility of hamstring in people with shortened hamstring. Methods: Thirty two subjects participated in this study. The subjects were assigned to either the Mulligan’s straight leg raise with traction technique group (n=16) or the hold-relax technique group (n=16). 90-90 straight leg raise test was performed for evaluation of hamstring shortening at initial time of study. After intervention, immediate effect (immediately after intervention) and lasted effect (60 min after intervention) were assessed. Results: In the both Mulligan’s straight leg raise with traction technique and hold-relax technique groups, significant improvements on flexibility of hamstring was observed in immediately and 60 minute after intervention (p<.05). However, there are no significant difference was observed between groups (p>.05). Conclusion: In the results of this study, both Mulligan’s straight leg raise with traction technique and hold-relax technique show immediately and 60 minute after evaluation effectiveness in flexibility of hamstring of people with shortened hamstring. In addition, although the difference is not statistically significant, Mulligan’s straight leg raise with traction technique was more increased average extension angle of knee joint at 90-90 straight leg raise test than the hold-relax technique.