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This paper proposes the optimal structure of a Printed Circuit Board-based micro-PCR Chip constructed on a PCB substrate using commercial adhesive tapes and plastic films. The proposed micro-PCR chip was composed of four layers. In a previous experiment, the chip had been made with a green PCB, yielding an unwanted reflection that prevented accurate analysis. In this study, the solder mask of the PCB substrate was coated black for a higher signal-to-noise ratio, and the area where the reaction chamber was attached was legend-printed with white silk for better reflection of the fluorescence light. The top cover, which was 180๐m thick, was also colored black to minimize the noise during fluorescence detection. As a result of carrying out PCR, it was found that the fluorescence brightness increased with increasing numbers of cycles.
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1. Introduction
2. Black PCB-Based Micro-PCR Chip
2.1. PCR Chip Driving System
2.2. The Proposed PCR Chip Structure
3. Experiments and Results
4. Conclusion
Acknowledgments
References