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The present study deals with the phyto-diversity, population structure including regeneration of un- established seedlings, established saplings and mature trees and physico-chemical properties of soil in southern moist mixed deciduous forest of Bori Wild Life Sanctuary in Madhya Pradesh, India. The results indicated that the average number of un-established and established regeneration of tree seedlings per hectare worked out to be 30,000 ha-1, and 2,778 ha-1, respectively, which were quite adequate. The dominant species in the stand were Tectona grandis, Saccopetalum tomentosum and Lagerstroemia parviflora. The distribution pattern of individuals of tree species in different girth classes was also seemed to be uninterrupted in the stand studied. This trend of uninterrupted distribution of in different growth phases with plenty of established regeneration was the healthy sign of establishment. Other associates showed different growth patterns. The dominant ground flora species were Achyranthes aspera, Zingiber roseum and Flemingia nana. The total density of tree and ground flora species was 247.37 ha-1 and 74,665 ha-1, respectively. The level of organic matter in soil was high, which was greater than 1.29 percent. The level of available nitrogen was low, which was less than 280 kg ha-1.