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This research was carried out to determine the differences of physiological activites between Grifola frondosa of log cultivation(LC) and Grifola frondosa of bottle cultivation(BC). Total flavonoids content, total phenolics content, electron donating ability(EDA), nitrite-scavenging ability(NSA), SOD-like activity and inhibitory effect of Xanthine oxidase were examined. The highest value of total flavonoid content is 5.96±0.81 mg/g in water extract from Grifola frondosa of log cultivation at 40oC (LCW40) but, one of total phenolics compound is 44.53±0.89 mg/g in water extract from Grifola frondosa of bottle cultivation at 40°… (BC-W40). The EDA using DPPH of BC-W40 extract showed the highest value of 97.14±0.71%. Nitrite-scavenging ability was 62.55°æ0.36% in extract from Grifola frondosa of BC-W40 at pH 1.2. The value was SOD-like activity showed the highest value of 18.95°æ1.39% in extract from LC-W40. Xanthine oxidase inhibitory activity was the highest value of 54.31±0.40% in extract from Grifola frondosa BC-W40, and dependent on concentrations. These results showed that a the antioxidant effects of Grifola frondosa is excellent. However, physiological activities of Grifola frondosa were not depend on caltivation method regulary, and were different according to kind of solvents, concentraitions and physialogical factors examined such as EDA, SOD-like activity and NSA.