원문정보
초록
영어
Mushroom have long been valued as highly nutritious and tasty foods in many societies throughout the world. It is known for biological activities including anti-inflammatory and anti-oxidative potential. However little is known about anticancer property. In this study, we investigated the anti-prostate cancer activity of mushrooms. For that, eight kinds of mushrooms such as, T. matsutake, S. crispa, G. lucidum-US, G. lucidum-AS, C. cardinalis-BR, G. frondosa, P. linteus, U. esculenta were extracted with hot water. Among them, three kinds of mushrooms including T. matsutake, G. lucidum-US and C. militaris-BR extracts inhibited prostate specific antigen (PSA) expression in prostate cancer cell, LNCaP. These results demonstrate that some of mushrooms inhibited PSA expression suggesting that the mushrooms might be a candidate for the treatment of prostate cancer.
목차
서론
재료 및 방법
버섯시료 추출 방법
세포 배양
RNA isolation
RT-PCR (Reverse Transcription-Polymerase Chain Reaction)
정량적 역전사 반응(Real-time quantitative polymerase chain reaction, qPCR)
PCR정량 분석
통계처리
결과 및 고찰
전립선암세포(LNCaP)에서 8종 버섯류 열수 추출물의 PSA mRNA 발현 억제효과
전립선암세포(LNCaP)에서 참송이버섯, 편각영지버섯및 현미동충하초버섯 열수 추출물의 PSA mRNA 발현 억제효과
적요
감사의 글
References