원문정보
초록
영어
Mushroom is known for anti-inflammatory and anti-oxidative potential. This study provides evidence that the inhibitory effect of mushroom on the expression of pro-inflammatory cytokines in human keratinocytes, HaCaT cells. To define the underlying mechanisms of action, tumor necrosis factorα/IFNγ-activated human keratinocytes model was used. Mushroom significantly inhibited the expression of cytokines in HaCaT cells. Taken together, the results demonstrate that mushroom inhibited inflammtion, suggesting that mushroom (DW extract: Grifola frondosa Cordyceps militaris), (Ethanol extract: Ganoderma lucidum, Lentinus edodes, Cordyceps militaris, Flammulina velutipes) might be a candidate for the treatment of skin inflammation.
목차
서론
재료 및 방법
버섯시료 추출
세포 배양
RNA isolation
역전사 반응(Reverse Transcription-Polymerase Chain Reaction, RT-PCR)
PCR정량 분석
통계처리
결과 및 고찰
피부각질세포(HaCaT)에서의 TNF-a/INF-g처리에 의한 열수 추출물의 TNF-a mRNA 발현 억제효과
피부각질세포(HaCaT)에서의 TNF-a/INF-g처리에 의한 에탄올 추출물의 TNF-a mRNA 발현 억제효과
적요
감사의 글
Reference