원문정보
Culture Optimization of Indigenous Microalgal Strain, Arthrospira sp. for Cosmetic Ingredients Screening
초록
영어
Microalgae including cyanobacteria are established commercial sources of high added-value raw materials such as β-carotene, astaxanthin, docosahexaenoic acid, eicosahexaenoic acid, phycobilin pigments and algal extracts for use in cosmetics. Arthrospira (Spirulina), a well known cyanobacterium, is rich in β- carotene, phycocyanin, gamma-linolenic acid (GLA) and most importantly, protein. Arthrospira research is scarce in Korea although extracts from this cyanobacterium is widely used in skin care products such as shampoo and cream but are imported. In this work, we isolated indigenous Arthrospira sp. from Korean fresh water. To optimize culture conditions, Photobiobox, a high throughput photobioreactor system for screening and optimizing microalgal culture conditions, was used. We showed that Arthrospira sp. had optimum temperature and light intensity of 25℃ and 200 μmol/m2/s, respectively, and the biomass concentration reached 1.45 g/L. The optimized culture condition can be used for biomass production and further studies on valuable algal bioproducts.
목차
Ⅰ. 서론
Ⅱ. 연구방법
1. 미세조류 분리 및 동정
2. 미세조류 배양 조건 탐색
3. 광생물 반응기를 이용한 바이오매스 생산
4. 유용물질 유도 스트레스 조건
5. 세포 물질 추출조건
Ⅲ. 연구결과 및 고찰
1. 분리 및 동정
2. 배양 최적화
3. Arthrospira sp. 바이오매스 생산
4. 세포 추출 최적화
Ⅳ. 결론
감사의 글
참고문헌