원문정보
The Evaluation of Antioxidant, Anti-inflammatory, and Anti-aging of Extract Solvent and Poria cocos by Parts
초록
영어
This study is for checking the possibility of Poria cocos as cosmetic materials. For this we carried out biological active evaluation about antioxidant, anti-inflammatory and anti-aging by using extraction parts of Poria cocos and solvent extraction. We extracted Poria cocos by extraction parts (bark, inside skin) and solvents (water extracts, ethanol), and then in order to evaluate antioxidant activity we processed samples by concentration and carried out DPPH radical scavenging and ABTS radical scavenging. Also, in order to evaluate effect of anti-aging we carried out evaluation of Elastase inhibitory activity and Collagenase inhibitory activity. To evaluate effect of anti-inflammatory we evaluated toxicity of samples through MTT assay with a macrophage (raw 264.7 cells) and measured Nitric Oxide production inhibitory activity. As a result at the experiment of DPPH radical scavenging and ABTS radical scavenging of antioxidant evaluation, ethanol extracts showed more superiority in all three groups, A, B and C than water extracts of Poria cocos of evaluations of the effect against wrinkles, according to the result of measurement of elastase inhibitory activity, PCE (A) showed 96.7% effect and PCE(B) showed 98.3% effect respectively at 1000μg/ml concentration. Also, according to the result of measurement of collagenase inhibitory activity, PCE (A) showed 60.0% effect and PCE (B) showed 72.2% effect respectively, so it means effect of ethanol extracts is superior. According to the result of nitric oxide production inhibitory activity, PCW (A) showed 60.4% effect and PCW (B) showed 60.3% effect respectively. This showed that effect of anti-inflammatory was greater in water extracts. In other words, ethanol extracts of Poria cocos proved effective against wrinkles by hindering antioxidant capacity and activities of elastase and collagenase by ROS. Water extracts showed superiority of effect of anti-inflammatory, so it suggests that Poris cocos extracts can be used as natural substance of cosmetics which are safe in antioxidant, anti-inflammatory, and anti-aging.
목차
Ⅰ. 서론
Ⅱ. 연구방법
1. 추출물의 제조
2. Electron donating abilities
3. ABTS+ radical cation 소거작용 측정
4. Elastase 저해활성 측정
5. Collagenase 저해활성 측정
6. 세포 배양
7. 세포 독성 측정
8. Nitric oxide (NO) 저해능 측정
Ⅲ. 연구결과 및 고찰
1. Electron donating abilities
2. ABTS+ radical cation activity
3. Elastase 저해활성 측정
4. Collagenase 저해활성
5. 세포 생존률 측정
6. Nitric oxide(NO) 저해능
Ⅴ. 결론
참고문헌
저자정보
참고문헌
- 1Studies on Improvement of Artificial Cultivation and Antioxidative Activity of Poria cocos네이버 원문 이동
- 2Research Paper : Studies on the Antimicrobial Effect of Herbal Extracts and It`s Cosmetic Application네이버 원문 이동
- 3Development of Advanced Functional Materials in Cosmetic Industry네이버 원문 이동
- 4Functional Cosmetic Effect of Porcine Placeta네이버 원문 이동
- 5Antioxidative Activity and Component Analysis of Phellinus linteus Extracts네이버 원문 이동
- 6Antioxidative and Biological Activity of Hot Water and Ethanol Extracts from Phellinus linteus네이버 원문 이동
- 7Quality and Functional Characteristics of Cultivated Hoelen (poria cocos Wolf) under the picking Date네이버 원문 이동
- 8Effectiveness of White Tea Water Extract on Skin Whitening Using in vitro Test네이버 원문 이동
- 9한국식품영양과학회지 논문투고규정 외네이버 원문 이동
- 10Beneficial Effect of Collagen Peptide Supplement on Anti-aging Against Photodamageearticle 원문 이동