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Human mitochondrial DNA analysis from canine feces was handled in this work. One woman committed suicide by hanging herself on door knob in her studio apartment. A part of her face skin was eaten by someone or something. Because of this case, ghostly rumors over a cannibal killer had been spread in the local society. But, it was estimated that her pet dog, Chihuahua, was referred to her broken face. The establishment of existence of human material in two lumps of canine dung and the identity test of this material with the dead were requested by the police. We used Micro Kit and Inhibit EX tablet in QIAamp DNA Stool Mini Kit for DNA purification. The DNAs were isolated from swabs rubbed lightly the exterior surface of dried canine feces, from hairs imbedded in dung, and from presumptive tissues that showed positive bloodstain test (leucomalachite green). Using direct sequencing analysis, the canine and human mitochondrial D-loop region could be acquired accurately from swabs and human D-loop region could be acquired from hairs imbedded in one lump of dung. The human D-loop region from swabs and hairs was identical to that from the blood of the deceased. Analysis result from presumptive tissues was not good but had partly identical haplotypes with the dead. D-loop region located in the human mitochondrial DNA was successfully acquired from canine feces. We could not get the dead woman’s short tandem repeat (STR) profile from canine feces. This is the first case report which detects human mitochondrial DNA from canine feces.