원문정보
초록
영어
Sampling biological evidence is one of the most critical step in forensic DNA typing. Touch DNA samples were known to be difficult to analyse compared to bloodstain, semen stain and saliva stains. In this study, nano filament fiber was evaluated as a tool for collecting trace DNA samples by comparing other commercial swabs such as cotton swab, cotton gauze, flocked swab, isohelix swab. Diluted blood stains were prepared on porous and non-porous materials such as wood and glass plate, respectively. DNA quantity was analyzed by qPCR and compared. Nano filament fiber showed higher concentration of DNA compared to other tools such as cotton swab. This study showed the feasibility of nano filament fiber as a novel tool for trace DNA analysis.
목차
I. 서론
II. 재료 및 방법
1. 시료 준비
2. 채취 도구 및 채취 방법
3. DNA 정제 및 정량
4. STR 분석
III. 결과 및 고찰
1. 혈흔 시료에서의 디엔에이 회수율 비교
2. 접촉 시료에서의 디엔에이 회수율 비교
IV. 결론
V. 참고문헌