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Objectives: This study is designed to evaluate the influential factors of career decision-making self-efficacy and career identity by health science college students on their job preparation beaviors. Methods: The total 714 questionnaires were distributed to juniors and seniors of health science college, and the result was statistically analyzed through IBM SPSS statistics 20.0. Results: 1. There was no significant difference of job preparation behavior between male students and female students, and while seniors showed highly active job preparation behaviors, those who had high satisfaction level toward their major studies, high academic achievement, and outgoing personality had highly active job preparation behaviors. 2. Students with high satisfaction level toward their major studies, high academic achievement, outgoing personality, and high expected salaries showed high career decision-making self-efficacy level. 3. Students with high satisfaction level toward their major studies, high academic achievement and highly outgoing personality showed high level of career identity. 4. Subsidiary factors of career decision-making self- efficacy were collection of job information, goal setting, planning, and self-assessment, and all of them showed correlation with job preparation behaviors. 5. Among subsidiary factors of career decision-making self-efficacy, “self-understanding” and understanding of job information showed correlation with job preparation behaviors. Conclusion: For improvement on career decision-making self-efficacy and career identity of health science college students, individual support, psychological support, educational support and other supports regarding job employment to provide wide paths and methods for job preparation behaviors are essential, and through various job employment education program and graded counseling, job preparation behaviors shall be promoted as a great strategy.