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The purpose of this study is to examine the possibility of anthocyanin pigments of Mulberry. In order to achieve this purpose, anthocyanin concentration was analyzed by measuring absorbency from anthocyanin 540 nm of Mulberry and color changes were observed according to changes in temperature and pH through color measurement. hair dyeing of anthocyanin pigments was found to very good at 40°C for 40 min. Unmordanted Hair dyeing was increased in dye intake (K/S) with increased dyeing temperature (20°C~60°C) and hair color was changed from red-brown to dark red-brown. anthocyanin pigments were specially sensitive to pH and dye intake was decreased with increased pH. Higher acidity generally led to a stronger reddish tint, while higher alkalinity led to a decrease in red coloration and a stronger yellow tint. For hair dyeing, mordanting methods (pre-mordanting, simultaneous mordanting, or post-mordanting) should be selected in consideration of the characteristics of mordants (dye intake, lightless, chroma and color). Based only dye intake (K/S), while pre -mordanting was effective for FeSO4, MgSO4, CaCO3, Na2CO3, simultaneous mordanting was effective for MgSO4, AlK (SO4)2, post-mordanting was effective for CuSO4, CH3COOH, Co (NO3)2. The rate of colorfastness was decreased with repeating shampoo washing. From these results, we can confirm that the extracts from Mulberry may be useful in the materials of hair coloring dye.