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The purpose of this study was to determine the assessment of multiple short-duration physical activity program for the body composition, blood lipid profiles and hemodynamic factors in elementary school children. The subjects were 52 children of the fifth grades of elementary school. physical activity group performed the combined physical activity program for 20 minutes/2time/day, 5 days/week, whereas control group were asked to maintain normal lifestyle during the same period. Measuring materials were body composition, blood lipid profiles(total cholesterol：TC, triglyceride：TG, low density lipoprotein cholesterol：LDL-C, and high density lipoprotein cholesterol：HDL-C), and hemodynamic factors (SBP：systolic blood pressure, DBP：diastolic blood pressure, HR：heart rate, C_stree index：cardiac stress index). From the results, positive changes were made in %body fat of body composition, TC, TG, and LDL-C levels of blood lipid profiles following 12 weeks multiple short-duration physical activity program. These results suggest that multiple short-duration physical activity program may be effective for helping elementary school children.