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The leaves of Perilla frutescens Britton var. japonica Hara were an annual and mint herb to have various potential functions. Phenolic components related to the antioxidant activity of multi-functions were analyzed for the quality control in P. frutescens leaves. Phenolic contents such as phenolic acid, cinnamic acid derivatives and flavonoids were shown from the methanol aqueous extracts using HPLC. The separation was performed on a reverse-phase C18 column using mobile phase composed of aqueous formic acid/acetonitrile mixtures at a flow rate of 0.5 mL·min-1. The detection was carried out on a DAD detector. This method was validated include selectivity, linearity, precision, accuracy, limit of detection and limit of quantitation.