약용식물의 건전종자 선발 및 발아율 검정을 위한 Tetrazolium Test 소개


Introducing for Healthy Seed Selection and Germination Test of Medicine Herbs using by Tetrazolium (2,3,5-triphenyl tetrazolium chloride) Test

이가연, 민지윤, 문병철, 강영민

피인용수 : 0(자료제공 : 네이버학술정보)



Propagation technology and evaluation of seed viability is a highly demanded technique in the production of Korean herbal medicine. To produce Raphanus seeds as the examples of Korean herbal medicine, seed germination improvement is very important. In this study, tetrazolium test was used because of rapid and simple method. After the selection of seed groups by the tetrazolium test, healthy seeds were A group and bad seeds were B group. The germination of seeds in A group were significantly different (alpha=0.05) from the germination of seeds in B group the given time. In addition, B seed group could not be cultured and propagated a long time because of tetrazolium chemical damage and had bad embryo physiologically. Also, nondestructive evaluation of viability for individual seeds as X-ray seed analysis or hyperspectral reflectance imaging system was required in future study. These seed analytical studies by tetrazolium test will be improved technology for reproductive medicinal herbs including Raphanus seeds.


 재료 및 방법
  1. 실험 재료
 결과 및 고찰
  1. TTC 발색법을 통한 종자활력 검정
  2. 선발된 불량종자와 건전종자의 기내 배양
 감사의 글


  • 이가연 Lee Kayoun. 한국한의학연구원 K-herb연구단
  • 민지윤 Min Jiyoon. 한국한의학연구원 K-herb연구단
  • 문병철 Moon Byeongcheol. 한국한의학연구원 K-herb연구단
  • 강영민 Kang Youngmin. 한국한의학연구원 K-herb연구단


자료제공 : 네이버학술정보

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