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RESEARCH ARTICLE

익모초 에탄올 추출물의 향장소재 연구

원문정보

A study on Cosmetic Applications of Ethanol Extracts of Leonurus sibiricus L.

김보애, 장영아

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

We conducted this study to investigate antioxidant and cytotoxicity of ethanol extracts of Leonurus sibiricus L. Also, we examined the cytoprotected effect of ethanol extracts of Leonurus sibiricus L. about antihemolytic of Sprague Dawley rat red blood cell. After extracting Leonurus sibiricus L. with ethanol. We treated samples by concentration and conducted DPPH radical scavenging activity and SOD-like activity. About human skin fibroblast HS68 cell, through MTT assay, we assessed toxicity of samples. Also, to assess effect of anti-hemolytic, we observed effect of extracts which we got after inducing oxidation of Sprague Dawley rat red blood cell using H2O2. The result showed that extracts of Leonurus sibiricus L. was effective as concentration of DPPH radical scavenging activity and SOD-like activity increased. After we checked cytotoxicity of Leonurus sibiricus L. using human fibroblast HS68 cell, it showed life rate more than 90 percent at 5~100 μg/ml and we made sure of its safety for skin. In the experiment for assessing of anti-hemolytic, we could observe the group that treated with extracts showed the higher rate of anti-hemolytic than control group that treated with H2O2 only. This result showed by eliminating ROS, extracts of Leonurus sibiricus L. could protect cell membrane and work as antioxidants. These results were that extracts of Leonurus sibiricus L. can eliminate active oxygen specie, have no cytotoxicity in fibroblast cell and show actual effectiveness of cell protection about anti-hemolytic activity in red blood cell, so they show possibility as functional natural product.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 실험동물
  2. 추출물의 제조
  3. Electron donating abilities
  4. SOD-like activity
  5. 세포배양
  6. 세포독성 평가
  7. 적혈구 부유액의 제조 및 용혈 측정
  8. 통계학적 분석
 Ⅲ. 연구결과
  1. Electron donating abilities
  2. SOD-like activity
  3. 세포 생존률 측정
  4. 항용혈 활성
 Ⅳ. 고찰
 Ⅴ. 결론
 참고문헌

저자정보

  • 김보애 Bo-Ae Kim. 목원대학교 생의약화장품학부
  • 장영아 Young-Ah Jang. 대구한의대학교 화장품약리학과

참고문헌

자료제공 : 네이버학술정보

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