earticle

영어

In order to analyze the anti-inflammatory effects of Centella asiatica (C. asiatica), which is effective in wound healing, blood circulation acceleration, fine line prevention, itchiness, and sedation, this study analyzed its anti-inflammatory effects in the macrophage of RAW264.7 mouse, and to find out its effects in scalp erythema improvement through clinical study, 11 people seen with scalp erythema were selected and conducted the study for a month from May 1~31, 2014. Study objects were divided into the experiment group using C. asiatica extract and comparison group not using C. asiatica and were instructed to spray about 2 mL twice a week, in the morning and at night, and their scalps were magnified 80 times using microscope (KONG PC Camera, Bomtech, Korea) and examined before experiment, 2 weeks later, and 4 weeks later. To analyze the anti-inflammatory effects of C. asiatica extract, RAW 264.7 cells were cultured in 37°C chamber at 5% CO2, and they were subcultured every 3 days to be processed for the same time with LPS. Cytotoxicity analysis was performed to find the proper concentration of C. asiatica extract, and the inflammatory factor, LPS 0.5 μg/mL was processed at 0 mg/mL, 0.01 mg/mL, 0.1 mg/mL, 1 mg/mL when processed at 1mg/mL the C. asiatica extract displayed 1.48 times the suppressive effect and was confirmed for anti-inflammatory effect. Clinical experiment was conducted to study the improvement effects of C. asiatica extract on scalp erythema on the experimental group using C. asiatica extract and the comparison group not using it, The improvement in the control group due to VES was 0.00 after 2 weeks and –6.67 after 4 weeks, which means that there was no improvement of erythema, and did not show a significant difference with over p<.05. Experiment group displayed the improvement rate of 33.33 after 2 weeks and 92.67 after 4 weeks; they displayed a great improvement of scalp erythema and displayed a significant difference with below p<.05. C. asiatica extract if released. Such results would influence in scalp improvement products, lead to more clinical studies using other various extracts for scalp improvements to contribute the development and utilization of scalp care products.