원문정보
Effect of Curcumin on the Adipose Tissue-derived Stem Cell Subculture
초록
영어
Adipose-derived stem cells (ASCs), which have characteristics of self-renewal and multipotency, are responsible for the tissue regeneration of the damaged tissue. Replicative senescence is the cause of an irreversible arrest of cell proliferation and loss cell function. Reactive oxygen species (ROS) are produced as a normal byproduct of aerobic life but the accumulation of the oxidative damage due to ROS induces the fundamental changes in cell aging process. In this study, the anti-aging effects of curcumin was studied in the long-term culture of ASCs by determining the expression of SA-β-galactosidase. Cell protection effect of curcumin was observed in UVB irradiated ASCs. Antioxidant, or anti-aging effect of curcumin by scavenging of intra-cellular ROS or enhancing the expression of antioxidant enzymes was also confirmed. These data suggest that curcumin may be effective in preventing the rise of oxidative stress during adipocyte differentiation through mechanisms that involves upregulation of endogenous antioxidant responses.
목차
Ⅰ. 서론
Ⅱ. 연구방법
1. 세포주 및 세포 배양
2. Neutral red assay
3. ROS assay
4. SA-β-galactosidase assay
5. RT-PCR (Reverse transcriptase polymerase chain reaction)
6. 통계 분석
Ⅲ. 연구결과 및 고찰
1. Neutral red assay
2. ROS assay
3. SA-β-galactosidase assay
4. 형태학적 관찰
5. 커큐민에 의한 항산화 효소 단백질 발현량 변화
Ⅳ. 결론
참고문헌