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Purpose: The purpose of this study was to investigate the influence of physical activity on smoking experience and smoking intensity in Korean high school students. Methods: This cross sectional study used secondary data from the 2013 Adolescent Health Behavior Online Survey. A total of 35,905 high school students were included in the study. Logistic regression was used for analysis of the influence of moderate and vigorous physical activity on smoking experience in a month and smoking intensity. Results: Adolescents with low (Adjusted OR: 1.242, 95% CI: 1.075~1.436) and moderate level (Adjusted OR: 1.094, 95% CI: 1.005~1.189) of moderate physical activity had more smoking experience than reference group, high level of physical activity. Also, smoking experience was more prevalent adolescents with low (Adjusted OR: 1.191, 95% CI: 1.064~1.333) and moderate level (Adjusted OR: 1.134, 95% CI: 1.039~1.237) of vigorous physical activity. Heavy smoking was more prevalent among adolescents who had low level of vigorous physical activity (Adjusted OR: 1.912, 95% CI: 1.347~2.712). Conclusion: Based on the results of this study, physical activity has significant influence on smoking experience and smoking intensity. Therefore, physical activity should be included smoking cessation and smoking prevention program for High School Students.