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The purpose of this study is to investigate the kinetics of reactive oxygen species and total antioxidant capacity during acute moderate treadmill running. Twelve healthy men ran treadmill at a intensity of 70% of the participant’s VO2max for 90 minutes. Blood samples were taken from finger in pre-exercise, 30 minutes, 60 minutes, 90 minutes of exercise for measuring the blood lactate, derivatives of Reactive Oxygen Metabolites (d- ROM), and biological antioxidant potential (BAP). During the treadmill running, respiratory gas analysis was also performed. independent t-test and one-way ANOVA were performed to compare the differences. The results were summarized as follows: 1. The differences between pre and post training were significant in reactive oxygen and total antioxidant capacity(p<.05) 2. The changes of reactive oxygen were significantly increased by aerobic training, and there were significant differences among exercise durations(p<.05). 3. The changes of total antioxidant capacity were not significant differences between training intensity groups(p>.05), and there were significant differences among exercise durations(p<.05). As shown above, the occurrence of reactive oxygen and total antioxidant were altered by exercise duration. In other words, the occurrence of reactive oxygen species were significantly increased in 30 minutes of exercise and total antioxidant capacity was significantly increased in 60 minutes of exercise. In conclusion, within 60 minutes of exercise might be suitable for the beginners in the point of the defending the cell against reactive oxygen by exercise.