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Purpose: The purpose of this study was to identify the factors influencing on practice of healthcare-associatedinfection control among clinical nurses. Methods: The subject of this study were 118 nurses who worked in medical·surgical ward and ICU of 2 general hospital in Gwangju city. Data were collected with a questionnaire. Datawere analysed using descriptive statistics, t-test, one-way ANOVA, Scheffé test, Pearson correlation and stepwisemultiple regression analysis using SPSS/WIN 12.0. Results: The major findings of this study were as follow: Therewere significant positive correlation between knowledge, recognition, empowerment and practice of healthcare-associated infection control. The significant factors influencing practice of healthcare-associated infectioncontrol were recognition and empowerment, which explained 68.8% of the practice of healthcare-associated infectioncontrol. Conclusion: These results indicate that recognition on healthcare-associated infection control andempowerment among clinical nurses should be reinforcement via consistent education, administrative and organizationalsupport at the level of hospital.