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Spring water traditionally has been used for hydrotherapy due to its useful minerals and therapeutic function through bathing and drinking. This study was conducted to confirm the long-term drinking effect of Gangcheon-mountain spring water (GSW) in ICR mice. The mice were randomly divided into control (n=18) and experiment (GSW; n=18) group, and were supplied with tab water (TW) and GSW for 100 days, respectively. As a result, fasting blood glucose concentration was 108.6±10.2 mg/dl in control group and 101.9±23.5 mg/dl in experiment group, and blood pressure (systolic/diastolic) was 121±25 / 84±24 mmHg and 123±27 / 74±19 mmHg in TW and GSW group, respectively. In the result of hematological test (complete blood cell count), there was no significant difference between two groups. The serum value of biochemical parameters were included in the normal range although Na and Cl revealed significant difference among them. The weight of organs and body did not show significance between two groups. The number of sperm in the experiment group (109.97±25.04) was higher than control (98.5±32.3) although it has no significance. These results indicate that GSW is safe as a daily drinking water. The difference of sperm number is speculated that drinking GSW has possibility of enhancement of reproductive function compared to TW due to useful minerals and comparatively low oxidation reduction potential value compared to TW.