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논문검색

Washing Culture Oil이 돼지 수정란의 체외 발육에 미치는 영향

원문정보

Effect of Washing Culture Oil on In Vitro Development in Porcine Embryos

이연주, 김유진, 이상희, 정희태, 양부근, 박춘근

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

The objective of this study was to investigate the changes of oxidative stress and antioxidant enzyme during in vitro development with washing culture oil in porcine embryos. During the culture, the four types of culture oil such as paraffin oil with or without washing and mineral oil with or without washing were examined. The oil was washed with PZM-3 during 7 days and collected oil only. The embryos were stained with CellTrackerTM Red, DCFDA and Hoechst 33342 to confirm the effects of the oil. As a results, Cleavage rates and total cell number were no difference among the four oil groups. However, ≥16 cell embryos were significantly different in fore type oil treatment and blastocyst rate was significantly higher washing paraffin treatment than in other group(p<0.05). Also, the expression of free radical were lower in washing paraffin oil than in other groups (p<0.05). On the other hand, the expression of glutathione were not significant different among paraffin oil with or without washing and mineral oil with or without washing, however washing paraffin oil and washing mineral groups were higher than other treatment groups. In conclusion, the washing oil was expected with positive effects on in vitro development in porcine embryos.

목차

ABSTRACT
 서론
 재료 및 방법
  Culture Oil의 준비
  난자의 준비
  난자의 체외성숙
  정자의 준비
  체외 수정 및 체외 배양
  Reactive Oxygen Species (ROS) 및 Antioxidant Ability측정
  배반포 염색
  실험 계획
 결과
 고찰
 인용문헌

저자정보

  • 이연주 Yeon-Ju Lee. 강원대학교 동물생명과학대학
  • 김유진 Yu-Jin Kim. 강원대학교 동물생명과학대학
  • 이상희 Sang-Hee Lee. 강원대학교 동물생명과학대학
  • 정희태 Hee-Tae Cheong. 강원대학교 수의과대학
  • 양부근 Boo-Keun Yang. 강원대학교 동물생명과학대학
  • 박춘근 Choon-Keun Park. 강원대학교 동물생명과학대학

참고문헌

자료제공 : 네이버학술정보

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