원문정보
초록
영어
To simplify the different biological investigation of the methanolic extract and solvent-solvent partitioning of Lablab purpures (L. purpures) bark. In-vitro anti-oxidant study was determined using total DPPH radical scavenging assay. In vitro antimicrobial study was measured by observing zone of inhibition. The cytotoxic activity was studied using brine shrimp lethality bioassay and thrombolytic activity by clot disruption method. The antioxidant potential was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Folin-Ciocalteau reagents using butylated hydroxytolune (BHT) and ascorbic acid as standards. The Aqueous soluble fraction revealed the highest free radical scavenging activity (IC50 = 48.76 μg/mL). The antimicrobial screening of the bark of L. purpures exhibited mild to moderate activity in test microorganisms. The CSF showed the maximum relative percentage inhibition against Salmonella parathyphi (34.2%) for bacteria and C. albicans (28.8%) for fungi whereas, lowest relative percentage inhibition against Sarcina lutea (22.0%) for bacteria and Aspergillus niger (24.4%) for fungi. In the brine shrimp lethality bioassay, The LC50 values of Carbon tetrachloride and N-Hexane soluble fraction were found 92.18 μg/mL, and 68.95 μg/mL respectively while the LC50 values of standard Vincristine sulphate was 1.37 μg/mL. The methanolic extract and its organic soluble fractions of Lablab purpureus at concentration 2.0 mg/mL, significantly protected the lysis of erythrocyte membrane induced by hypotonic solution and heat as compared to the standard, acetyl salicylic acid (0.10 mg/mL). The MSF and AQSF produced 61.48 % and 53.75% inhibition of hemolysis of RBC caused by hypotonic solution respectively, whereas acetyl salicylic acid (0.10 mg/mL) showed 76.42%. Ethanol extract of L. purpures and all of its different partitions exhibited moderate thrombolytic activity of 37.25%-2.40%. Very good preliminary screening and simplified experiments were able to show the different biological activity of methanolic extract and its soluble fractions of L. purpures at a time.
목차
1. Introduction
2. Experimental Section
2.1. Plant Materials and Extraction
2.2. Chemicals
2.3. Test Microorganisms
2.4. Free Radical Scavenging Activity Measured by 1,1-Diphenyl-2-picryl-hydrazyl (DPPH)
2.5. In vitro Thrombolysis Activity
2.6. Brine Shrimp Lethality Bioassay
2.7. Antimicrobial Activity
2.8. Determination of Relative Percentage Inhibition
2.9. Preparation of Erythrocyte Suspension
2.10. Heat Induced Haemolysis
2.11. Hypotonicity Induced Haemolysis
2.13. Statistical Analysis
3. Results and Discussion
3.1. Antioxidant Activity
3.2. Thrombolysis Activity
3.3. Toxicity Studies
3.4. Anti-microbial Activity
3.5. Membrane Stabilization Effects
4. Conclusions
References