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Notes

큰느타리버섯의 고온적응성 형질에 관련된 SCAR Marker 개발

원문정보

Development of strain-specific SCAR marker for selection of Pleurotus eryngii strains adaptable to high-temperature

김수철, 김혜수, 박소연, 류재산, 조수정

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

In this study, SCAR marker that differentiates Pleurotus eryngii strains adaptable to high-temperature from control strain was developed. Genomic DNAs of 7 control strains of Pleurotus eryngii and 7 Pleurotus eryngii strains adaptable to hightemperature were analyzed by bulked segregant analysis (BSA) using randomly amplified polymorphic DNA (RAPD). Onehundred twenty RAPD primers were screened on bulked DNA samples and a unique DNA fragment with the size of 385 bp was yielded by OP-A06 primer from the Pleurotus eryngii strains adaptable to high- temperature. A sequence characterized amplified region (SCAR) marker, designated as OP-A06-1-F and OP-A06-1-R, was designed on the basis of the determined sequence. The PCR analysis with the OP-A06-1 primer showed that this SCAR marker can clearly distinguish the Pleurotus eryngii strains adaptable to high-temperature from the control strains.

목차

ABSTRACT
 서론
  대립 계통과 고온성 계통 선발
  RAPD를 이용한 bulked segregant analysis
  SCAR 마커 디자인
 적요
 감사의 글
 참고문헌

저자정보

  • 김수철 Su Cheol Kim. 경남과학기술대학교 제약공학과
  • 김혜수 Hye Soo Kim. 경남과학기술대학교 제약공학과
  • 박소연 So Yeon Park. 경남과학기술대학교 제약공학과
  • 류재산 Jae-San Ryu. 경상남도농업기술원 친환경연구과
  • 조수정 Soo Jeong Cho. 경남과학기술대학교 제약공학과

참고문헌

자료제공 : 네이버학술정보

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