Research Article

Paenibacillus polymyxa CK-1이 생산한 길항물질이 Trichoderma sp. 생육에 미치는 영향


The effect of antagonists produced by Paenibacillus polymyxa CK-1 on the growth of Trichoderma sp.

이상원, 최진상, 김철호

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The separation of the bacteria inhibiting Trichoderma sp. mold, the strain causing blue mold disease that occurs frequently when cultivating mushroom while carrying out the efficient fermentation of mushroom medium, from the growth was done. In about 200 strains isolated primarily from fungus garden samples, 6 strains were secondly isolated, which had fast growth rates and a clear zone on the plate medium of SM, AM, and CM. Among the 6 strains isolated, the C-1 strain showed high enzymatic activity of cellulase, amylase, and protease, and strong antibacterial activity for the T. virens and T. harzianum, selected finally. The selected C-1 strain was identified as Paenibacillus polymyxaby the result of the identification by Bergey's Manual of Systematic Bacteriology and the analysis of the nucleotide sequence of 16S rRNA, and named as P. polymyxa CK-1. In reviewing the growth conditions of the P. polymyxa CK-1 strain, the optimum cultivation temperature was 45oC, and the optimum pH for growth was in the range of 6.0~7.0. Appropriate incubation time of P. polymyxa CK-1 for the growth inhibition of the fungus T. virens and T. harzianum was 22 to 36 hours. And the fungal growth was not observed, even when leaving two molds inoculated on each petri dishes, which were treated with 24 hour culture solution of P. polymyxa CK-1 strain for 10 days. As a result of studying the thermal stability of the antagonists produced by the P. polymyxa CK-1 strain, no mycelial growth of the two fungi was observed in the test group treated for 20 minutes at 60oC and 100oC, but mycelial growth was slightly observed in the test group treated for 20 minutes at 121oC. As aresult of reviewing the impact of the P. polymyxa CK-1 culture medium on mushroom mycelial growth, it showed no effect on a variety of mushroom mycelial growth including enoki mushroom and shiitake mushroom.


 재료 및 방법
  미생물의 분리 및 동정
  분리균주의 길항활성
  분리균주의 생육특성
  배양 시간에 따른 길항물질의 생산
  길항물질의 열 안정성
  분리균과 버섯과의 생육
 결과 및 고찰
  길항세균의 분리
  분리균주의 동정
  분리균주의 생장 특성
  배양 시간에 따른 길항물질의 생산
  길항물질의 열 안정성
  P. polymyxa CK-1이 버섯균사 생육에 미치는 영향
 감사의 글


  • 이상원 Sang-Won Lee. 경남과학기술대학교 제약공학과
  • 최진상 Jin-Sang Choi. 경남과학기술대학교 식품과학부
  • 김철호 Chul-Ho Kim. 경남과학기술대학교 제약공학과


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