원문정보
초록
영어
Basigin/EMMPRIN/CD147 has been reported to be associated with inflammatory diseases and cancers. Detailed insight into this multifunctional protein in the context of cellular functions may provide a key to its pathophysiological role in many diseases. Here, we investigated the potential role of basigin in activation of the NF-B and AP-1 signaling cascades associated with the MyD88 and TRIF adaptor proteins. MyD88- and TRIF-dependent activation of NF-B signaling pathway was inhibited by basigin-specific antibody and siRNA. In addition, induction of TRIF-dependent, but MyD88-independent, AP-1 activation was inhibited by basigin-specific siRNA. Taken together, these results suggest that basigin plays a key regulatory role in activation of MyD88- and TRIF-mediated cell signaling pathways, and provide an important insight into basigin-mediated cellular processes that can serve as a guide for the development of new anti-inflammatory therapeutics via intervention in basigin-mediated cellular functions.
목차
1. Introduction
2. Materials and Methods
2.1. Flow cytometry
2.2. Immunoblotting
2.3. Luciferase reporter assay
2.4. Small interfering RNA (siRNA)-directed basigin knockdown
3. Result
3.1. Effect of basigin on activation of the MyD88-dependent NF-B signaling pathway
3.2. Effect of basigin on activation of the TRIF-dependent NF-B signaling pathway
3.3. Effect of basigin on activation of the TRIF-dependent AP-1 signaling pathway
4. Discussion
Acknowledgements
References