원문정보
초록
영어
During pregnancy, diabetes mellitus exerts detrimental effects on the development of the fetus, especially the central nervous system. In the current study, we evaluated the effects of postnatal treadmill exercise on short-term memory in relation with cell proliferation and apoptosis in the hippocampus of rat pups born to streptozotocin (STZ)-induced dia-betic maternal rats. Adult female rats were mated with male rats for 24 h. Two weeks after mating, the pregnant female rats were divided into two groups: control group and STZ injection group. The pregnant rats in the STZ injection group were administered 40 mg/kg of STZ intraperito-neally. After birth, the rat pups were divided into the following four groups: control group, control with postnatal exercise group, maternal STZ-in-jection group, and maternal STZ-injection with postnatal exercise group. The rat pups in the postnatal exercise groups were made to run on a treadmill for 30 min once a day, 5 times per week for 2 weeks beginning 4 weeks after birth. The rat pups born to diabetic rats were shown to have short-term memory impairment with suppressed cell proliferation and increased apoptosis in the hippocampal dentate gyrus. Postnatal treadmill exercise alleviated short-term memory impairment by increased cell proliferation and suppressed apoptosis in the rat pups born to dia-betic rats. These findings indicate that postnatal treadmill exercise may be used as a valuable strategy to ameliorate neurodevelopmental prob-lems in children born to diabetics.
목차
INTRODUCTION
MATERIALS AND METHODS
Animals and treatments
Exercise protocols
Determination of short-term memory
Tissue preparation
BrdU immunohistochemistry
Caspase-3 immunohistochemistry
Bax immunohistochemistry
TUNEL staining
Data analysis
RESULTS
Effect of postnatal treadmill exercise on memory in the step-down avoidance task
Effect of postnatal treadmill exercise on the number of BrdU-positive cells in the hippocampal dentate gyrus
Effect of postnatal treadmill exercise on the number of caspase-3-positive cells in the hippocampal dentate gyrus
Effect of postnatal treadmill exercise on the number of Bax-positive cells in the hippocampal dentate gyrus
Effect of postnatal treadmill exercise on the number of TUNEL-positive cells in the hippocampal dentate gyrus
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES