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단보

잠재지문 현출 시약이 유전자감각에 미치는 영향

원문정보

Effect of latent fingerprint reagents on DNA Profiling

이현주, 박희연, 유제설, 임시근

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

Identification of latent fingerprint in crime scene is one of the most important thing for crime scene investigators. Lots of fingerprint identification methods have been developed to be applied to various kinds of evidence. Nowadays, forensic DNA typing technology showed incredible sensitivity of successful result even from fingerprint. In this study, six kinds of fingerprint identification methods including Ninhydrin, Ninhydrin with heat, DFO, iodine, RTX and CA were examined regarding to their effect to DNA profiling by quantitative analysis of purified DNA using quantitative PCR. The fingerprint chemicals were treated onto the diluted bloodstain on paper following purification of DNA after 1, 3, 5 and 7 days. RTX treatment resulted approximately 70 % decrease in DNA concentration showing the most destructive or inhibitory chemicals. Ninhydrin with heat treatment and ninhydrin only treatment showed approximately 50 % and 30 % decrease, respectively. DFO, Iodine and CA showed relatively little effect on DNA concentration of 10 % to 20 % decrease. The effect of fingerprint chemicals on forensic DNA typing was also demonstrated by STR analysis. Latent fingerprint identification and DNA typing are equally important forensic tools. It is necessary to establish a guideline for selection and sequence of latent fingerprint identification method for successful DNA typing from fingerprint.

목차

Abstract
 I. 서론
 II. 재료 및 방법
  2.1 잠재 지문 현출 시약의 제조 및 검사 방법
  2.2 DNA
 III. 결과 및 고찰
  3.1 시간 경과에 따른 DNA 정량 분석
  3.2 지문현출시약이 DNA에 미치는 영향
  3.3 STR 분석
 IV. 참고문헌

저자정보

  • 이현주 Hyun Ju Lee. 순천향대학교 법과학대학원 법과학과, 순천향대학교 법과학연구소
  • 박희연 Hee Yeon Park. 국립과학수사연구원 남부분원 법의학과 유전자분석실
  • 유제설 Je Seul Yu. 순천향대학교 법과학대학원 법과학과, 순천향대학교 법과학연구소
  • 임시근 Si Keun Lim. 국립과학수사연구원 남부분원 법의학과 유전자분석실

참고문헌

자료제공 : 네이버학술정보

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