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RESEARCH ARTICLE

3T3-L1 세포에서 Adipogenesis 억제를 통한 Kaki-tannin의 항비만 활성

원문정보

Anti-obese Activities of Kaki-tannins Prepared from Various Persimmons through Inhibition of 3T3-L1 Adipogenesis

손명수, 신유진, 이승철, 김교남

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

Persimmon (Diospyros kaki Thunb.) is widely cultivated in Asian countries such as Korea, China, and Japan. Although its various beneficial functions have been well-documented, its anti-obese effect is relatively unknown. Here, we prepared kaki-tannins from unripe astringent Daebong persimmon (DU), red-ripe non-astringent Daebong persimmon (DR), unripe astringent Cheongdo flat shaped persimmon (CU), red-ripe non-astringent Cheongdo flat shaped persimmon (CR), and unripe non-astringent Fuyu persimmon (FU). Cytotoxicity of the kaki-tannins were evaluated by MTT assay in 3T3-L1 cells. The treatment up to 100 μg/mL of all kaki-tannins for 24 h did not affect to the 3T3-L1 cell viability. Thus, the concentration up to 100 μg/mL of the kaki-tannins were chosen for subsequent experiments. The treatment with 100 μg/mL of kaki-tannins prepared from DR and CU significantly inhibited lipid accumulation of 3T3-L1 adipocytes. In line with this result, the decreased number and size of intracellular lipid droplets in 3T3-L1 adipocytes treated with DR and CU kaki-tannins were observed. The 3T3-L1 cells treated with DR and CU kaki-tannins showed down-regulated mRNA expression levels of C/EBPα and FAS as judged by RT-PCR. Our study also revealed that DR and CU kaki-tannins-inhibited adipogenesis are strongly limited in the early stage of 3T3-L1 adipogenesis. These evidences suggest that kaki-tannin has the potential as anti-obese natural materials, and different function and role of kaki-tannins in anti-adipogenic activities may be mediated to persimmon types and maturation stage.

목차

Abstract
 Ⅰ. 서론
 Ⅱ. 연구방법
  1. 실험재료
  2. Kaki-tannin의 제조
  3. Kaki-tannin의 세포독성
  4. 3T3-L1 세포 배양 및 지방세포 분화
  5. Kaki-tannin의 adipogenesis 억제활성 평가
  6. 현미경 관찰을 통한 kaki-tannin의 지방축적 억제활성 평가
  7. Reverse transcriptional-PCR (RT-PCR) 분석을 통한 유전자 발현 분석
  8. 통계분석
 Ⅲ. 연구결과 및 고찰
  1. Kaki-tannin의 세포독성 평가
  2. Kaki-tannin의 adipogenesis 억제활성
  3. 지방세포의 수 및 크기 관찰
  4. RT-PCR를 통한 mRNA 발현수준 평가
  5. 분화 기간별 kaki-tannin 처리에 따른 adipogenesis 억제활성
 Ⅳ. 결론
 참고문헌

저자정보

  • 손명수 Myung-Soo Shon. 경남대학교 식품생명학과
  • 신유진 Yu-Jin Shin. 경남대학교 식품생명학과
  • 이승철 Seung-Cheol Lee. 경남대학교 식품생명학과
  • 김교남 Gyo-Nam Kim. 경남대학교 식품생명학과

참고문헌

자료제공 : 네이버학술정보

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