원문정보
The Extract of Chlorella vulgaris Exerts Protective Effects Against Ultraviolet B Radiation-induced Damages in Human Dermal Fibroblasts
초록
영어
Ultraviolet B (UVB) radiation accelerates skin aging by inducing direct DNA damage and indirect intracellular damage, which induce cell cycle arrest and apoptosis in human dermal fibroblasts (NHDFs). In this study, we demonstrated that pretreatment with the hexane extract of Chlorella vulgaris microalgae protects UVB-mediated cell damages in NHDFs. Firstly, we compared the cytotoxicity effect of four extracts (ethanol, hexane, ethyl acetate and distilled water) of Chlorella vulgaris on NHDFs, and found that the four extracts had a little cytotoxicity at 1-20 μg/ml. Additional biochemical assay showed that UVB-induced cytotoxicity was significantly reduced in NHDFs posttreated with only hexane extract of Chlorella vulgaris. Furthermore, to increase the UVB-protection effect of the extract, we changed the concentration and treatment time, and found that 48 h of posttreatment with hexane extract (20 μg/ml) of Chlorella vulgaris significantly increased the cell viability by 34.01±5.86% compared with the viability of UVB-irradiated NHDFs. In addition, we found that the hexane extract of Chlorella vulgaris promoted the DNA repair properties in NHDFs, as revealed by luciferase-based DNA repairing assay. Overall, our results indicate that hexane extract of Chlorella vulgaris is implicated as a potential chemopreventive agent through UVB protection effect on NHDFs.
목차
Ⅰ. 서론
Ⅱ. 연구방법
1. Chlorella vulgaris 추출물
2. 진피섬유아세포 구입 및 배양
3. 세포 자외선 조사
4. 세포 생존율(cell viability) 조사
5. Luciferase 의존적 DNA손상 평가실험
Ⅲ. 연구결과 및 고찰
1. Chlorella vulgaris 추출물의 세포독성 평가
2. Chlorella vulgaris 추출물의 자외선보호 및 자외선에 의한 세포손상 복구 효능 평가
3. Chlorella vulgaris 추출물의 세포 효능 극대화
4. Chlorella vulgaris 추출물의 자외선에 의한 DNA 손상 복구 능력 평가
Ⅳ. 결론
감사의 글
참고문헌